Shuler Kargi Bioprocess Engineering Free Pdf.zip


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A new book is on the way that will possibly revolutionize the way you think about the history of science. Written by Richard Ambrosini, PhD, an award winning historian of science,. A new book is on the way that will possibly revolutionize the way you think about the history of science. Written by Richard Ambrosini, PhD, an award winning historian of science,.Treatment of Edwardsiella ictaluri-mediated disease in channel catfish with 2-deoxy-D-glucose. Edwardsiella ictaluri infection of channel catfish (Ictalurus punctatus) was used as an experimental model to determine if induction of the glycolytic enzyme phosphofructokinase (PFK) could influence the outcome of disease. Injection of E. ictaluri into channel catfish caused severe ulcerative disease in channel catfish within two weeks. Total live weight gains of catfish used in the experiments was decreased by approximately 50%. Fish injected with E. ictaluri plus 2-deoxy-D-glucose (2-DG) had 40% lower salinities compared to fish injected with E. ictaluri alone. The salinities of fish injected with E. ictaluri plus 2-DG was decreased to a similar level as for fish injected with E. ictaluri and supplemented with NaHCO3 (pH 10.5) indicating an inability of these fish to regulate salinity. Trypan blue staining of the gills and spleen of E. ictaluri-infected catfish indicated that the disease was not due to capillary plugging. Histology of the kidney confirmed infection of the medulla. Fish injected with E. ictaluri plus 2-DG had a 99% mortality rate compared to the 40% mortality rate for fish injected with E. ictaluri alone. Immunological analyses indicated that 2-DG produced no effect on systemic humoral immunity. However, catfish injected with E. ictaluri and 2-DG had 10-fold less PFK activity in the liver compared to controls. A similar phenomenon of decreasing PFK activity was seen in catfish injected with E. ictaluri and pyruvate (PYR) as a control for the injection. The results indicate that addition of 2-DG or PYR to E. ictaluri-

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