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e). The negative control amplification with DNA from the mock sample was also carried out to check for contaminations. The numbers of *E. coli* plasmid DNA in the samples were also checked by amplifying the plasmid DNA using specific primers for pUC18 (Supplementary Table [S1](#MOESM1){ref-type=»media»}). Evaluation of detection limits {#Sec15} —————————— The calculated values of the standard curves and limits of detection of the *E. coli* plasmid DNA were determined by using the least significant difference (LSD) at 95% confidence level. Detection limits for real samples were determined by the correlation coefficient R^2^ and slope of the standard curves. The experimental design and performance of the proposed biosensor {#Sec16} —————————————————————— An experimental design was carried out to study the combined effect of the three important parameters including DNA target concentration, target hybridization time, and the hybridization temperature on the performance of the proposed biosensor. A set of three different concentrations of the *E. coli* plasmid DNA (25, 50, and 100 pg/µL) were prepared in a 1X TBE buffer (6 M urea, 0.015 M TBE, and 1 M NaCl). The hybridization times (30, 60, 90, and 120 min) were considered at each concentration, and the temperature (25, 45, and 60 °C) was fixed to check the combined effect of the hybridization time and temperature on the performance of the proposed biosensor. Under these conditions, a total of 54 different combinations of the target concentrations, hybridization times, and hybridization temperatures were analysed (Fig. [2](#Fig2){ref-type=»fig»}). Detection of *E. coli* plasmid DNA using the biosensor {#Sec17} —————————————————— A 25 µL of each concentration of the *E. coli* plasmid DNA sample were diluted in a 1X TBE buffer. A 200 µL aliquot of the diluted target DNA samples were transferred into the microfluidic channel. Then, the real sample was run in the flow channel of the microfluidic chip at three different temperatures (25, 45, and 60 °



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Live, on-screen diagramming with a mouse or pen, or digital «brainstorming» on the fly. Draw a diagram, sketch a circuit, or simply explore your ideas on your screen. Start with a blank drawing for any project, or pick a drawing from your background with new «in» and «out» points. Quickly «connect» to a drawing, grid or section, or reference object, to open it directly. (video: 4:55 min.) Automatic Geometric Dimensioning and Management: Take advantage of Autodesk® 360 technology to track, model, store, and document your designs to enable a streamlined CAD workflow. Easy communication: Share and collaborate within CAD. Create views of drawings for better project collaboration. Import Autodesk® 360 plans and 3D models to your drawings. Key benefits: Take advantage of Autodesk® 360 technology to track, model, store, and document your designs to enable a streamlined CAD workflow. Take advantage of Autodesk® 360 technology to track, model, store, and document your designs to enable a streamlined CAD workflow. Easily create views of drawings for better project collaboration. Easily create views of drawings for better project collaboration. Enable accurate documentation with the ability to create rich, multimedia files. Explore: Create, modify, or view models and data for complex mechanical designs. Explore and create freeform shapes, add any profile, or surface any extrusion. Explore and create freeform shapes, add any profile, or surface any extrusion. Expanded features for designing for precision. Achieve tight tolerances with easy-to-use commands to accurately align parts in your drawing. Achieve tight tolerances with easy-to-use commands to accurately align parts in your drawing. Fully customizable commands. You can create your own commands or customize common commands to suit your workflows and increase efficiency. You can create your own commands or customize common commands to suit your workflows and increase efficiency. New innovations for CAD. Use the new Cylinder option to replace multiple extrusions with a single extrusion. Reduce the number of loops and surfaces when you use the Circle command to make shapes. Use the new Cylinder option to replace multiple extrusions with a single extrusion. Reduce the number of loops and surfaces when you use the Circle command to make shapes


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