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. 4shared Driver Driver updates and software 2/2 (3) 2/1 (4). istream4w64 bluetooth.ET737.ev7 vechi driver free download torrent 4shared hamilton 4426 ver.1.0. 4shared 4shared Maocony Tv Card DriverCloning, expression and characterization of a novel γ-glutamyltranspeptidase from maize. γ-Glutamyltranspeptidase (γ-GT) is a key enzyme for the synthesis and metabolism of glutathione (GSH), which is the major non-protein thiol in eukaryotic cells and an important reducing agent for detoxification of xenobiotics and oxidative stress. Here, we cloned and characterized a novel γ-GT gene, designated as Maotanxi18γ-GT gene (Ma18γ-GT gene), from maize (Zea mays L.). The sequence analysis indicated that Ma18γ-GT gene was 2768 bp in length, consisted of a 1456-bp coding region and a 392-bp upstream non-coding sequence. The predicted polypeptide contained 457 amino acids (aa) and comprised a putative signal peptide of 21 aa (M20 to M40), an α-helix region of 108 aa and four pairs of cysteines. The sequence analysis indicated that Ma18γ-GT gene belonged to the putative γ-GT sub-class of glutathione S-conjugating enzymes. Bioinformatic analysis revealed Ma18γ-GT gene had two splicing sites and possessed two typical γ-GT domains. Ma18γ-GT gene was predominantly expressed in maize roots, leaves, silk and flowers, and weakly expressed in midveins, epidermis and vascular bundle of stems. Ma18γ-GT mRNA level was increased under high-salinity stress in maize leaves. Ma18γ-GT protein was localized in the cytosol of maize root hair and cells of the lateral root cap and epidermis of the root. Ma18γ-GT activity in Arabidopsis (Arabidopsis thaliana L.) was increased under high-salinity stress, and was recovered by treating with GSH. Taken together, these results indicated that Ma18γ-GT gene played a pivotal role in the detoxification of high-salinity stress.// // Gener 595f342e71
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